Agilent Technologies 1100 Series Bedienerhandbuch PDF herunterladen (Seite 72)

62 1100 Series Capillary LC System Manual

4 Basic System Troubleshooting

Failure to produce peaks, or abnormally small peaks, after injection

Symptoms:

There are no peaks, or the peak size is significantly below the typical peak size

for this method with this column.

Failure to produce peaks, or abnormally small peaks, after injection: Possible Causes and Suggested Actions

Possible Causes Suggested Actions Notes

A leak in a sample carrying

area of the system.

• Using a flashlight and absorbent tissue,

carefully check for leaks in the following areas:

a. All micro-sampler valve ports.

b. The junction of the needle and sample loop

capillary.

c. The needle/seat interface.

d. Column inlet and outlet.

e. Flow cell inlet capillary, at the capillary/cell

body junction.

At very low flow rates, a leak may

never accumulate enough liquid to

trigger the module leak sensors. Low

flow rate leaks are also very hard to

see.

The 40 µl chamber of the

micro-sampler metering head

has developed bubbles.

• In the user interface diagnostics, access the

Change Piston function of the micro-sampler

maintenance positions. This function draws the

metering piston fully inward, clearing the

chamber.

• Under flow conditions, activate this function for

at least 5 minutes. The sampler valve must be in

the mainpass position at this time. Any bubbles

which have formed in the chamber will now be

cleared by the flow.

In most applications, only a small

part of the available 40µl metering

head volume is used. At very low

flows, bubbles may form in the

unused space between the metering

piston and the chamber wall. The

bubbles act to defeat the draw of

sample into the needle.

For best results in clearing bubbles,

the mobile phase being pumped

should not contain water.

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