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26 1100 Series Capillary LC System Manual

1 Installing your Capillary LC System

Inject the Check-out Sample

The purpose of the instrument check is to demonstrate that all modules of the

instrument are correctly installed and connected. It is not a test of the

instrument performance.

A single injection of the Agilent Technologies isocratic test sample (Agilent

part number 01080-68704) is made under the method conditions given below:

For system configurations other than those shown above, the method

conditions might need to be altered to produce the desired chromatogram.

Tabl e 4 Method conditions for injecting a test sample

Flow: 15.0 µl/minute

Stoptime: ~7.00 minutes

Solvent A: 30% (HPLC grade Water)

Solvent B: 70% (HPLC grade Acetonitrile)

Wavelength DAD/MWD: Sample: 254/4 nm, Reference: 360/80 nm

Injector Volume: 200 nl

Column Temperature 25.0 °C or ambient

Agilent 1100 Series

Capillary LC Instrument

Degasser

Capillary pump - 20 µl/minute sensor installed

Micro Autosampler

Column Compartment - optional

Detector - DAD with 500 nL flow cell installed

ChemStation

Column: ZORBAX SB C18, 5 µm, 150 x 0.5 mm

Agilent Part No. 5064-8256

Standard: Agilent Part No. 01080-68704

0.15 wt.% dimethylphthalate, 0.15 wt.% diethylphthalate

0.01 wt.% biphenyl, 0.03 wt.% o-terphenyl in methanol

Diluted 1:10 in Acetonitrile

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Agilent Technologies 1100 Series Bedienerhandbuch Seite 36