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30 1100 Series Capillary LC System Manual

2 Optimizing Performance

Hints for Successful Use of the Capillary Pump

Pump issues

• Flush the pump extensively. First with in the purge mode, second with a

pressure applied to remove all the gas bubbles. It is recommended to do this

first with 100% A and than 100%B.

• The system pressure must be higher than 20 Bar at the pump outlet.

• In micro mode abnormally high column flow variations are an indication of

dirt within the system, blocked filters or loose pump valves.

• Place solvent cabinet with the solvent bottles always on top (or at a higher

level) of the capillary pump.

• Prevent blocking of solvent inlet filters (never use the pump without solvent

inlet filter). Growth of algae should be avoided.

• When using buffer solutions, flush the system with water before switching

it off.

• Check the pump plungers for scratches when changing the piston seals.

Scratched plungers will lead to micro leaks and will decrease the lifetime of

the seal.

• After changing the plunger seals, perform the seal wear-in procedure. See

the pump reference manual.

• Place the aqueous solvent on channel A and the organic solvent on

channel B. The default compressibility and flow sensor calibration settings

are set so. Always use the correct calibration values.

• For generation of fast gradients on short columns remove the mixer, enter

the new pump configuration and select the fast gradient range for the

primary flow rate (chromatographic performance will not be affected).

• When running the micro mode check the correct instrument setup (flow

sensor type, used mixer and filter).

• Make sure to observe the minimum recommended flow setpoint:

• Normal mode100 µl/min

• Micro mode, 20 µl flow sensor: 1 µl/min

• Micro mode, 100 µl flow sensor:10 µl/min

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Agilent Technologies 1100 Series Bedienerhandbuch Seite 40