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1100 Series Capillary LC System Manual 41

Optimizing Performance 2

How to Optimize the Compressibility Compensation Setting

The compressibility compensation default settings are 50 × 10

-6

/bar (best for

most aqueous solutions) for pump head A and 115 × 10

-6

/bar (to suit organic

solvents) for pump head B. The settings represent average values for aqueous

solvents (A side) and organic solvents (B side). Therefore it is always

recommended to use the aqueous solvent on the A side of the pump and the

organic solvent on the B side. Under normal conditions the default settings

reduce the pressure pulsation to values (below 1 % of system pressure) that

will be sufficient for most applications. If the compressibility values for the

solvents used differ from the default settings, it is recommended to change the

compressibility values accordingly. Compressibility settings can be optimized

by using the values for various solvents described in Table 6 on page 42. If the

solvent in use is not listed in the compressibility table, when using premixed

solvents and if the default settings are not sufficient for your application the

following procedure can be used to optimize the compressibility settings:

1 Start channel A of the capillary pump with the adequate flow rate. The

system pressure must be between 50 and 250 bar

2 Before starting the optimization procedure, the flow must be stable. Use

degassed solvent only. Check the tightness of the system with the pressure

test.

3 Your capillary pump must be connected to an Agilent ChemStation or an

Agilent 1100 control module, the pressure and%-ripple can be monitored

with one of these instruments,

otherwise connect a signal cable between the pressure output of the

capillary pump and a recording device (for example, 339X integrator) and

set parameters.

Zero 50 %

Att 2^3

Chart Speed 10 cm/min

4 Start the recording device with the plot mode.

NOTE

Use the capillary pump in the Normal Mode at least 100 µl/min.

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Agilent Technologies 1100 Series Bedienerhandbuch Seite 51