
46
Setting up an Analysis
Preparing the HPLC System
6 Purge the pump. For more information see “Priming and Purging the System”
on page 41.
7 Allow the detector at least 15 minutes to provide a stable baseline.
8 For the isocratic pump, fill the solvent bottle with the mixture of HPLC-grade
bidistilled water (30 %) and acetonitrile (70 %).
9 Click on the Load Method button and select
'()B/&0and press OK. The
default LC method parameters are transferred into the 1100 modules.
Figure 24 Loading Default LC Method
refer to step 10 “Setting
Parameters” on page 47
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