Agilent Technologies 1100 Series Betriebsanweisung PDF herunterladen (Seite 45)

Agilent 1100 Series Valves 39

Valve Applications 4

Sample enrichment and Sample stripping

For the sample enrichment phase the valve is switched to position 1. The

eluent Pump A transfers the injected sample onto the enrichment column. The

sample is retained and enriched on this column, whereas the sample matrix is

flushed into the waste. At the same time the second eluent pump B is

equilibrating the analytical column.

After the valve is switched to position 2 pump B is backflushing the sample on

the analytical column for separation and subsequent detection by LC/MS or

optical detectors.

Agilent Technologies offers capillary kit for sample enrichment and sample

stripping. The content is listed in Table 11 on page 40 and Table 12 on page 41.

Sample stripping

Sample stripping is another method to remove complex sample matrices. In

difference to sample enrichment the matrix is trapped on the pre-column

while the analytes pass through onto the analytical column.

Figure 22 Sample stripping (schematic setup)

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Agilent Technologies 1100 Series Betriebsanweisung Seite 45