Agilent Technologies DP235 Betriebsanweisung PDF herunterladen (Seite 236)

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Contents ▲ 236 ▼ Index

Histogram evaluation

The two histograms displaying the results of the assay are related to calcein (blue

fluorescence) and annexin V (red fluorescence). High fluorescence values in the blue

histogram indicate living cells, low values correspond to dead cells. See the following

image as an example.

The values are displayed in the result table, each histogram has its own table:

Low fluorescence value

indicates dead cells

High fluorescence value

indicates living cells

All measured events

All events in relation to the

blue marker (here calcein)

Living cells in relation to all measured cells (high calcein fluorescence)

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