Agilent Technologies DP235 Betriebsanweisung PDF herunterladen (Seite 164)

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Contents ▲ 164 ▼ Index

Generating Dot Plots

Single events can also be displayed related to both fluorescence values, generating a

map of dot plots. In dot plot view, the events (cells with a minimum fluorescence

intensity) are displayed in a coordinate system (logarithmic axis scaling). Each axis

represents a fluorescence color. A high number of events (cells) with similar

fluorescence values are displayed as a dense cluster of dots, as shown in the following

image.

To determine the number of cells whose blue and red fluorescence lies within a defined

range, you can insert regions. Additionally, a gate can be set for either red or blue

fluorescence to obtain percentual values on a defined population.

As a cell passes through the detector, its blue

and red fluorescence values are measured.

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